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Lyudmil S. Antonov    Ph.D in Biology

Publications

1. Atanassov, I., Russinova, E., Antonov, L., and A. Atanassov. 1998. Expression of an anther-specific chalcone synthase-like gene is correlated with uninucleate microspore development in Nicotiana sylvestris. Plant Molecular Biology, 38: 1169-1178 [PubMed Entry] [Y14507] [Y14506]

2. Todorovska, E.G., Atanassova, A., Antonov, L.S., and K.P. Dudov. 1997. Studies on the mouse rpL32 pseudogene family: Features of a new member. Biological Chemistry, 378(12):1531-1535 [PubMed Entry] [AF065390]

3. Todorovska, E.G., Dudov, K.P., and L.S. Antonov. 1996. RFLP-s in human genome detected by human ribosomal protein cDNA probe. Biotechnology and Biotechnological Equipment, 10(2-3): 10-17

4. Chakarska, D.D. and Antonov, L.S. 1995. Application of intuitionistic fuzzy sets in plant tissue culture and in vitro selection. Notes on IFS, 1(1):70-73 Notes on Intuitionistic Fuzzy Sets

5. Antonov, L.S., Dudov, K.P. and A.I. Atanassov. 1995. Site-specific inactivation of bacterial genes involved in plant soft rot. Biotechnology and Biotechnological Equipment 9(1):14-28

6. Vayda, Michael E., Antonov, L.S., Yang, Z., Butler, William O., Lacy, George H. 1992. Hypoxic stress inhibits aerobic wound-induced resistance and activates hypoxic resistance to bacterial soft rot. American Potato Journal 69:239-253

7. "Molekulite na zhivota (The Molecules of Life)", Translation from Scientific American (1987) 41, Nauka i Izkustvo Publishers, 1990, Sofia, Bulgaria.

8. Antonov, L.S. and K.G. Milanov. 1983. Antibodies to myelin basic protein in multiple sclerosis: a long-term study. Bull. INPN (Bulg), 27:65-68.

9. Milanov, K.G., L.S. Antonov and Z. Manolova. 1983. Microcomplement fixation with myelin basic protein as a test for multiple sclerosis. Bull. INPN (Bulg), 27:69-72.

10. Antonov, L.S. and K.G. Milanov. 1982. Inhibition of synaptic acetylcholineesterase by antibodies found in sera of schizophrenic patients. J. Neurol. Psych. Neurosurg. (Bulg), 6:105-108.

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Abstracts

Plant Mol Biol 1998 Dec;38(6):1169-78 Related Articles,Nucleotide, Protein, Books, LinkOut

Expression of an anther-specific chalcone synthase-like gene is correlated with uninucleate microspore development in Nicotiana sylvestris.

Atanassov I, Russinova E, Antonov L, Atanassov A.

Institute of Genetic Engineering, Kostinbrod, Bulgaria. ivanatonassov@mailexcite.com

Two cDNA clones, specifically expressed in Nicotiana sylvestris anthers during uninucleate microspore development, were isolated using a subtractive hybridization approach. Sequence analysis showed that one of them, NSCHSLK, displayed a high level of similarity to several anther-specific chalcone synthase-like (CHSLK) proteins and an ORF from chromosome I of Arabidopsis thaliana. A lower, but significant, similarity to chalcone synthases and closely related enzymes (CHSRE) was also detected. The structure of the nschslk gene was found to be typical of the chalcone (chs)/stilbene (sts) synthase family. Expression of NSCHSLK mRNA was confined to microspores and tapetal cells. UV-irradiation or infection with Phytophthora parasitica var. nicotianae of transgenic Nicotiana benthamiana plants carrying a chimeric nschslk/GUS gene indicated that the nschslk promoter exhibits the same anther-specific, developmentally regulated expression pattern. Comparison of CHSRE and CHSLK polypeptide sequences revealed some important similarities and differences between the two groups. The data presented in this study, suggest that the anther-specific chslk genes represent a separate sub-family of plant polyketide synthases related to chs/sts in terms of gene structure, polypeptide sequence and the possible catalytic mechanism, but differing in substrate/product specificity. The putative role of CHSLK enzymes in anther development and particularly in exine synthesis is discussed.

PMID: 9869422 [PubMed - indexed for MEDLINE]

Biol Chem 1997 Dec;378(12):1531-5 Related Articles,Nucleotide,Books, LinkOut

Studies on the mouse rpL32 pseudogene family: features of a new member.

Todorovska EG, Atanassova A, Antonov LS, Dudov KP.

Institute of Genetic Engineering, Kostinbrod, Bulgaria.

A new processed pseudogene (rpL32-5C) that belongs to the mouse rpL32 multigene family has been sequenced and compared to previously characterised mammalian rpL32 family members. rpL32-5C has intact direct terminal repeats of 13 nt, a poly A tail of 10 nt and it does not contain conserved promoter elements at its 5' end. The comparison with the L32 cDNA and 4A processed pseudogene shows a deletion of 25 nt in rpL32-5C as well as several nt substitutions that lead to frameshifts and interruption of the ORF. Most of the consistent substitutions occur in the third codon position lending support to the suggestion that all rpL32 processed pseudogenes have been derived from transcripts of the functional gene.

PMID: 9461352 [PubMed - indexed for MEDLINE]

Published sequences

Y14507. Nicotiana sylvest...[gi:2326771] GenBank Entry
LOCUS       NSCHSLKA1               3300 bp    mRNA    linear   PLN 24-FEB-1999
DEFINITION  Nicotiana sylvestris CHSLK gene.
ACCESSION   Y14507
VERSION     Y14507.1  GI:2326771
KEYWORDS    anther-specific protein; chalcone synthase-like gene.
SOURCE      wood tobacco.
  ORGANISM  Nicotiana sylvestris
            Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
            Spermatophyta; Magnoliophyta; eudicotyledons; core eudicots;
            Asteridae; euasterids I; Solanales; Solanaceae; Nicotiana.
REFERENCE   1  (bases 1 to 3300)
  AUTHORS   Atanassov,I., Russinova,E., Antonov,L. and Atanassov,A.
  TITLE     Expression of an anther-specific chalcone synthase-like gene is
            correlated with uninucleate microspore development in Nicotiana
            sylvestris
  JOURNAL   Plant Mol. Biol. 38 (6), 1169-1178 (1998)
  MEDLINE   99084767
REFERENCE   2  (bases 1 to 3300)
  AUTHORS   Atanassov,I.I.
  TITLE     Direct Submission
  JOURNAL   Submitted (07-AUG-1997) I.I. Atanassov, Institiute of Genetic
            Engineering, 2232 Kostinbrod-2, BULGARIA
FEATURES             Location/Qualifiers
     source          1..3300
                     /organism="Nicotiana sylvestris"
                     /db_xref="taxon:4096"
                     /clone="G3"
                     /tissue_type="anther"
                     /clone_lib="lambda DASHII cDNA library"
                     /dev_stage="tetrad to uninucleate stages"
     exon            1375..1534
                     /number=1
     gene            join(1423..1534,1826..2805)
                     /gene="NSCHSLK"
     CDS             join(1423..1534,1826..2805)
                     /gene="NSCHSLK"
                     /codon_start=1
                     /product="anther-specific protein"
                     /protein_id="CAA74847.1"
                     /db_xref="GI:2326772"
                     /db_xref="SPTREMBL:O24625"
                     /translation="MGKAFPAQLVPQDCLVEGYIRDTNCQDLAIKEKLERLCKTTTVK
                     TRYTVMSKEILDKYPELATEGTPTIKQRLEIANPAVVEMAKQASQACIKEWGRSAEEI
                     THIVYVSSSEIRLPGGDLYLATELGLRNDIGRVMLYFLGCYGGVTGLRVAKDIAENNP
                     GSRVLLTTSETTILGFRPPNKARPYDLVGAALFGDGAAAVIIGTEPIMGKESPFMELN
                     FATQQFLPGTNNVIDGRLTEEGINFKLGRDLPEKIQDNIEEFCKKIIAKADLREAKYN
                     DLFWAVHPGGPAILNRLENTLKLQSEKLDCSRRALMDYGNVSSNTIFYVMEYMREELK
                     NKKNGGEEWGLALAFGPGITFEGILLRSL"
     intron          1535..1825
                     /gene="NSCHSLK"
                     /number=1
     exon            1826..2991
                     /number=2
BASE COUNT     1110 a    604 c    580 g   1006 t
ORIGIN      
        1 gaattccttt aaatattcct tttatactaa atatggcaac tgaatattat tttttaacga
       61 atcaaaaatt aaaaaacaaa tctgtaacac ctctatacat aaaattgcat ccaccaaatg
      121 ctactatcta taaactaaac attgatgggt cttgctcatc tacccagcaa aatatggtat
      181 tggtggagtt tttcgaaact accaaggcaa ctggatcatg agatttgcag gtaaatccaa
      241 tccaggcgca aatgttcaga cagaactcct tgcactactc atgggattga aacttgttgt
      301 gcaacaatat ctcaacccca tcatcattga gacagatgca catgcaatca tcggtatgtt
      361 taactcaact actatgcact acactaatct cattaacgat tgcaggttat tactcctaca
      421 gctggatagc cctcctatac aatacatcta caaagggcaa aattgtgttg cagatagttt
      481 agccaaacat gaagtcatgc atgcacagga gagctgcata ctttgtggga gaccatcatc
      541 ttttgcagag ccttcttacc accaagatca tatcgacacg ctacaaagga ggttcgttac
      601 aatcaactcc agctaactca acacaacgct ctttgctcta actactttgt gtaatagtat
      661 tgttctatct agtactaata gtagtgttct atctagtact aactatcttt cttgtaatga
      721 ttcatacggg gtctttgtag taaactctag cttagtaact atgatgcctc ttatgtatct
      781 tgtaaactgt tctcatgtac ctggttaatt ccctataata taagtacatc ttttcgacaa
      841 aaaaagaaga agaacaacaa cattttagca atgaaaaata agaagacaaa cacaacacat
      901 ttatagaatt aacatttgaa caatgactag tggtgcagct agttggtgca ttaccctctt
      961 tgccccctcc cccgcgcata aaaaaatcta aaaaataata tcagtaaaaa gaaagaaaaa
     1021 aacaaaagga agtgtgaaaa gtgaaaagac tgctttcgtt ttatttatac tttcacacaa
     1081 acaagccaca agaatcaatc ataatatatt aaaatctgca acactcttaa agcatgcact
     1141 agtttttata atttgtcatg ccagaagttc tcttaacaca attataccaa cctttctcca
     1201 actacagatt tagctctcca tttatatttg atgcaataac atgtttgaaa caatctcata
     1261 ctaagatttc atcagacaac agcaaatctc aagtcacatt ctcttagctt ccatatttta
     1321 tccaatatgt cacagaacgg caagaacatt aatggtgcat ccaagtactt ttttcagccg
     1381 tcgacgcgcc ttcctactcc aggaaaagcc accattcttg ccatgggcaa agccttccct
     1441 gcacaacttg tccctcagga ttgcttggtt gaaggctaca ttcgcgatac caactgtcaa
     1501 gatttggcta ttaaggagaa gttagagcgc ctttgttaag caaaaattta cttccattaa
     1561 ttattgttgc atgctgatga tcctgacctt tttcatgaat ttaacttgta tatactaaca
     1621 atataaagac tttttaccac tatcaacgta ttttaaacat gctgtaggta aaaagtctaa
     1681 ttttccacat tacaagtcca acttttgttg gacacctgta gttatttttg atgatatgat
     1741 tgtgtgaaaa actcttttga ctgtcagtgt atataagtta aactttttaa aacatttcta
     1801 gtgacttttc catgtttttc tgtaggtaaa actactactg tgaagaccag atatacagtg
     1861 atgtcaaaag aaattttgga caagtatcca gaactagcaa ctgaaggcac accaacaatc
     1921 aaacaaaggc tagaaattgc aaatccagca gttgtggaaa tggcaaaaca agcaagccaa
     1981 gcttgcatta aggaatgggg aagatcagca gaggaaatca cacacattgt gtatgtttcc
     2041 tccagcgaaa tacgtttacc aggaggtgat ctttaccttg caactgagct tggattgagg
     2101 aatgacattg gacgcgtaat gctgtatttt ttgggttgct atggtggtgt cacaggcctt
     2161 agagttgcca aagatatagc tgaaaataat ccagggagca gagttctatt gacaacttct
     2221 gagaccacaa ttcttggttt taggccacca aataaggcta gaccatatga tcttgttggt
     2281 gctgcacttt tcggtgatgg agctgctgct gttataattg gaactgaacc aattatggga
     2341 aaagaatctc ctttcatgga attgaatttt gcaactcaac aattcttgcc agggacaaat
     2401 aatgtgatcg atgggcgact tactgaagaa gggataaact tcaaattagg aagggacctg
     2461 cctgagaaaa ttcaggacaa cattgaggaa ttttgcaaga aaattatagc taaggctgat
     2521 ttgagggaag ctaagtacaa tgacttgttc tgggctgttc atccaggcgg accggctata
     2581 cttaacagat tggagaacac actgaagtta caaagtgaga aactggattg tagcagaagg
     2641 gcattgatgg attatggaaa tgtgagtagc aatactatat tctatgtgat ggagtatatg
     2701 agggaagagt tgaagaataa gaaaaatggt ggtgaagaat ggggacttgc tttagcattt
     2761 ggccctggca tcacttttga aggcattctc cttaggagtc tctaaatgtc atgttatgat
     2821 attaggttgt tcattttctt tgtactgtcc ttagacagaa caaaattgtt tcactgtttt
     2881 tttcctttcc taaatacgaa ggtaaaataa gagacatgag ttcacaactc atgcaatttt
     2941 agttaaagtt actggaacta tggaagaatg tcatatgata aatgaaagaa aattaatgga
     3001 gaaatgattt aatttatcca ctatggtgtg aacatgcttg tttatgcatt cagcataatc
     3061 ttactagctc tcacatgtta ctcacctatt tctatctagg atacatatga tatttaatat
     3121 atgtgttaaa tactactagc atcaacatat aaaaacaaac caagtaatgg acatatacat
     3181 ctttattctt ttggatgtgg tattcaatca gaaaaaatta atttggccaa aaaattaaag
     3241 taaaaaggat ttatttaccc ttttaggaaa aaagtctata tcaaaactca aaaatgtttt
//
Y14506. Nicotiana sylvest...[gi:2326773] GenBank Entry
LOCUS       NSCHSLKG3               1326 bp    mRNA    linear   PLN 24-FEB-1999
DEFINITION  Nicotiana sylvestris CHSLK gene.
ACCESSION   Y14506
VERSION     Y14506.1  GI:2326773
KEYWORDS    anther-specific protein; chalcone synthase-like gene.
SOURCE      wood tobacco.
  ORGANISM  Nicotiana sylvestris
            Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
            Spermatophyta; Magnoliophyta; eudicotyledons; core eudicots;
            Asteridae; euasterids I; Solanales; Solanaceae; Nicotiana.
REFERENCE   1  (bases 1 to 1326)
  AUTHORS   Atanassov,I., Russinova,E., Antonov,L. and Atanassov,A.
  TITLE     Expression of an anther-specific chalcone synthase-like gene is
            correlated with uninucleate microspore development in Nicotiana
            sylvestris
  JOURNAL   Plant Mol. Biol. 38 (6), 1169-1178 (1998)
  MEDLINE   99084767
REFERENCE   2  (bases 1 to 1326)
  AUTHORS   Atanassov,I.I.
  TITLE     Direct Submission
  JOURNAL   Submitted (07-AUG-1997) I.I. Atanassov, Institiute of Genetic
            Engineering, 2232 Kostinbrod-2, BULGARIA
FEATURES             Location/Qualifiers
     source          1..1326
                     /organism="Nicotiana sylvestris"
                     /db_xref="taxon:4096"
                     /clone="NSANT1"
                     /tissue_type="anther"
                     /clone_lib="anther, plasmid and lambda gt10 cDNA library"
                     /dev_stage="tetrad to uninucleate stages"
     mRNA            1..1326
                     /gene="NSCHSLK"
     gene            1..1326
                     /gene="NSCHSLK"
     CDS             49..1140
                     /gene="NSCHSLK"
                     /codon_start=1
                     /product="anther-specific protein"
                     /protein_id="CAA74846.1"
                     /db_xref="GI:2326774"
                     /db_xref="SPTREMBL:O24625"
                     /translation="MGKAFPAQLVPQDCLVEGYIRDTNCQDLAIKEKLERLCKTTTVK
                     TRYTVMSKEILDKYPELATEGTPTIKQRLEIANPAVVEMAKQASQACIKEWGRSAEEI
                     THIVYVSSSEIRLPGGDLYLATELGLRNDIGRVMLYFLGCYGGVTGLRVAKDIAENNP
                     GSRVLLTTSETTILGFRPPNKARPYDLVGAALFGDGAAAVIIGTEPIMGKESPFMELN
                     FATQQFLPGTNNVIDGRLTEEGINFKLGRDLPEKIQDNIEEFCKKIIAKADLREAKYN
                     DLFWAVHPGGPAILNRLENTLKLQSEKLDCSRRALMDYGNVSSNTIFYVMEYMREELK
                     NKKNGGEEWGLALAFGPGITFEGILLRSL"
BASE COUNT      422 a    230 c    310 g    364 t
ORIGIN      
        1 cagccgtcga cgcgccttcc tactccagga aaagccacca ttcttgccat gggcaaagcc
       61 ttccctgcac aacttgtccc tcaggattgc ttggttgaag gctacattcg cgataccaac
      121 tgtcaagatt tggctattaa ggagaagtta gagcgccttt gtaaaactac tactgtgaag
      181 accagatata cagtgatgtc aaaagaaatt ttggacaagt atccagaact agcaactgaa
      241 ggcacaccaa caatcaaaca aaggctagaa attgcaaatc cagcagttgt ggaaatggca
      301 aaacaagcaa gccaagcttg cattaaggaa tggggaagat cagcagagga aatcacacac
      361 attgtgtatg tttcctccag cgaaatacgt ttaccaggag gtgatcttta ccttgcaact
      421 gagcttggat tgaggaatga cattggacgc gtaatgctgt attttttggg ttgctatggt
      481 ggtgtcacag gccttagagt tgccaaagat atagctgaaa ataatccagg gagcagagtt
      541 ctattgacaa cttctgagac cacaattctt ggttttaggc caccaaataa ggctagacca
      601 tatgatcttg ttggtgctgc acttttcggt gatggagctg ctgctgttat aattggaact
      661 gaaccaatta tgggaaaaga atctcctttc atggaattga attttgcaac tcaacaattc
      721 ttgccaggga caaataatgt gatcgatggg cgacttactg aagaagggat aaacttcaaa
      781 ttaggaaggg acctgcctga gaaaattcag gacaacattg aggaattttg caagaaaatt
      841 atagctaagg ctgatttgag ggaagctaag tacaatgact tgttctgggc tgttcatcca
      901 ggcggaccgg ctatacttaa cagattggag aacacactga agttacaaag tgagaaactg
      961 gattgtagca gaagggcatt gatggattat ggaaatgtga gtagcaatac tatattctat
     1021 gtgatggagt atatgaggga agagttgaag aataagaaaa atggtggtga agaatgggga
     1081 cttgctttag catttggccc tggcatcact tttgaaggca ttctccttag gagtctctaa
     1141 atgtcatgtt atgatattag gttgttcatt ttctttgtac tgtccttaga cagaacaaaa
     1201 ttgtttcact gtttttttcc tttcctaaat acgaaggtaa aataagagac atgagttcac
     1261 aactcatgca attttagtta aagttactgg aactatggaa gaatgtcata tgataaatga
     1321 aagaaa
//
AF065390. Mus musculus clon...[gi:3152699] GenBank Entry
LOCUS       AF065390                 750 bp    DNA     linear   ROD 23-MAY-1998
DEFINITION  Mus musculus clone rpL32-5C ribosomal protein L32 (Rpl32)
            pseudogene, complete sequence.
ACCESSION   AF065390
VERSION     AF065390.1  GI:3152699
KEYWORDS    .
SOURCE      house mouse.
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Rodentia; Sciurognathi; Muridae; Murinae; Mus.
REFERENCE   1  (bases 1 to 750)
  AUTHORS   Todorovska,E.G., Atanassova,A., Antonov,L.S. and Dudov,K.P.
  TITLE     Studies on the mouse rpL32 pseudogene family: features of a new
            member
  JOURNAL   Biol. Chem. 378 (12), 1531-1535 (1997)
  MEDLINE   98121250
REFERENCE   2  (bases 1 to 750)
  AUTHORS   Todorovska,E.G., Atanassova,A., Antonov,L.S. and Dudov,K.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (14-MAY-1998) Molecular Genetics, Institute of Genetic
            Engineering, Kostinbrod-2 2232, Bulgaria
FEATURES             Location/Qualifiers
     source          1..750
                     /organism="Mus musculus"
                     /strain="BALB/c"
                     /db_xref="taxon:10090"
                     /clone="rpL32-5C"
     repeat_region   113..125
                     /note="direct 5' terminal repeat"
     gene            126..616
                     /gene="Rpl32"
                     /note="Rpl32-5C processed pseudogene; ribosomal protein
                     L32"
                     /pseudo
     repeat_region   617..629
                     /note="direct 3' terminal repeat"
BASE COUNT      261 a    161 c    160 g    168 t
ORIGIN      
        1 cgatctgatg ccctcttctg gtgtgtctga aagaagtgtg aacagtgtac cacatacata
       61 aaataaataa ataaatcttt aaaaagaaaa gaatagtaat aagaaatatt aagactataa
      121 agtgagcttc ttcctcggcg ccgcctacga ggtagctgcc gtctgttttg cagcatcatg
      181 gctgccctct ggcctctggt gaagctcaag atcgtcaaaa agaggaccaa gaagttcatc
      241 agacaccagt cagaccgata tgtgaaaatt aagcgaaact ggcagaaacc cagaggttca
      301 aaagccagat cctgatgccc aacatcggct acaggagcaa caagaaaacc aagcacacgc
      361 tgcccagcgg cttccgcaag ttcctggtcc acagtgtcaa tgagctggaa gtgttgctga
      421 tgtgcaacaa atcttactgt gctgagattg ctcataatgt gtcctctaag aaccgaaaat
      481 ccatcctaga aagagcagca cagctggcca tcagagtcac caatcccaac gctaggctat
      541 gcaccgaaga aaaagaatag atggcaagtg tgcatgtttt gtgtttaaat aaaatcacaa
      601 aaactgaaaa aaaaaagact ataaagtgaa tctttaaaga aattacttgt tttactttag
      661 tataccagtg ttctgcagat acgcactaca tgcagtaccc aaagaagcca aaagatggca
      721 atagaacttc tggaactggg agtgcaggca 
//
K02060. Mus musculus ribo...[gi:3228365] GenBank Entry
LOCUS       MUSRPL3A                5380 bp    DNA     linear   ROD 06-AUG-1998
DEFINITION  Mus musculus ribosomal protein L32-3A (3A) gene, complete cds.
ACCESSION   K02060
VERSION     K02060.1  GI:3228365
KEYWORDS    .
SOURCE      house mouse.
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Rodentia; Sciurognathi; Muridae; Murinae; Mus.
REFERENCE   1  (bases 1 to 5380)
  AUTHORS   Dudov,K.P. and Perry,R.P.
  TITLE     The gene family encoding the mouse ribosomal protein L32 contains a
            uniquely expressed intron-containing gene and an unmutated
            processed gene
  JOURNAL   Cell 37, 457-468 (1984)
  MEDLINE   84205680
REFERENCE   2  (bases 1135 to 4892)
  AUTHORS   Antonov,L.S.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-1993) Molecular Genetics, Institute of Genetic
            Engineering, Kostinbrod-2, Bulgaria
REFERENCE   3  (bases 1 to 5380)
  AUTHORS   Antonov,L.S.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JUN-1998) Molecular Genetics, Institute of Genetic
            Engineering, Kostinbrod-2, Bulgaria
  REMARK    Nucleotide sequence updated by submitter
COMMENT     On Jun 16, 1998 this sequence version replaced gi:200780.
            The expressed gene 3A, is probably the only L32 gene in the myeloma
            cell line, MPC 11, that contains introns.
FEATURES             Location/Qualifiers
     source          1..5380
                     /organism="Mus musculus"
                     /strain="BALB/c"
                     /db_xref="taxon:10090"
                     /clone="rpL32-3A; pL32; pL33"
                     /cell_type="sperm"
     repeat_region   112..233
                     /rpt_family="B1"
                     /rpt_type=dispersed
     repeat_region   272..445
                     /rpt_family="B2"
                     /rpt_type=dispersed
     repeat_region   812..823
                     /rpt_family="B1"
                     /rpt_type=terminal
     repeat_region   824..967
                     /rpt_family="B1"
                     /rpt_type=dispersed
     repeat_region   970..981
                     /rpt_family="B1"
                     /rpt_type=terminal
     prim_transcript 1503..4728
                     /gene="3A"
     mRNA            join(1503..2428,3066..3247,4552..4728)
                     /gene="3A"
                     /product="ribosomal protein L32-3A"
     gene            1503..4728
                     /gene="3A"
     intron          1539..2327
                     /gene="3A"
                     /note="A"
     CDS             join(2333..2428,3066..3247,4552..4681)
                     /gene="3A"
                     /codon_start=1
                     /product="ribosomal protein L32-3A"
                     /protein_id="AAC28897.1"
                     /db_xref="GI:200781"
                     /translation="MAALRPLVKPKIVKKRTKKFIRHQSDRYVKIKRNWRKPRGIDNR
                     VRRRFKGQILMPNIGYGSNKKTKHMLPSGFRKFLVHNVKELEVLLMCNKSYCAEIAHN
                     VSSKNRKAIVERAAQLAIRVTNPNARLRSEENE"
     exon            2333..2428
                     /gene="3A"
                     /number=2
     intron          2429..3065
                     /gene="3A"
                     /note="B"
     exon            3066..3247
                     /gene="3A"
                     /number=3
     intron          3248..4551
                     /gene="3A"
                     /note="C"
     exon            4552..>4681
                     /gene="3A"
                     /number=4
     repeat_region   4941..4958
                     /rpt_family="B1"
                     /rpt_type=terminal
     repeat_region   4959..5115
                     /rpt_family="B1"
                     /rpt_type=dispersed
     repeat_region   5116..5133
                     /rpt_family="B1"
                     /rpt_type=terminal
BASE COUNT     1253 a   1268 c   1404 g   1446 t      9 others
ORIGIN      406 bp upstream of HinfI site.
        1 catacccaat tcrgcatttg tccccacctc ctgaggccac tgtcaggatc accggtggca
       61 tctcttctgt ctgrgtttgt acaargtttg aaaatttttt aatttggtct gctgggcagt
      121 ggtgacacct tacatctcag cacttgggag gcagagacag gaagatctcc ggccagccta
      181 gtctacagag tgaattcgac cacagtcagg cttacacaga gaaaccctgt cttaaaacaa
      241 aacaaaacaa aacaaagggt gggactgggg tggggctgga gagatggctc tgcagttaag
      301 aacactgatt gctcttccag aggtctcggt tcaattccca ttaaccacat ggtgactcac
      361 aaccatctgt aatgggatct gatgccctct tctggtgtgt ctgaagacag ctacagtgtg
      421 ctcatataca taaatcttta aaaaatgaga tttattctat gtgattgagt gttttgcctg
      481 catgtatgag tatgtaccac atgcatgtag cgcctacaga aaagggcatc tgatccccta
      541 gggctggtgt taacaaggtt gtggaagcaa cgcttggttc ctggaactaa atgccagtcc
      601 tcttgaaaga gcaacagtgc tccaccaagc cacctcccca gctcaggytt ggyttaytcc
      661 tcctyagatt aytgagttty cacaatgacc tgtaaaacaa atgataagca aaatagacta
      721 aactcgacct acctctgctt cccaagtgct gttattaaaa aatgtgcacc accacaccca
      781 gccaatagac tattttctta gcaatattat caacctatca cttttttttt ttcgaaacag
      841 ggtttctctg tgtagctctg gctgtcctgg aaccctctct gtagctcagg ctggcctcga
      901 actcagaaac ccgcctgcct ctgcctccca agtgcgggga ttaaaggcat gagccaccac
      961 cgcccggcta acccatcact tattaataca ttaggtacat accataagta gagaagattt
     1021 aaacatacag aagtcgcgag gaggctcagt agcacttcat aagcaggggt ttgcacatct
     1081 gtatattagg aagttttagg acatttgctc aaccaaccgc ctaaggatct tcgaagagtc
     1141 tctgttttgc tacctcccag cctcaaggag gcaccctcgg gaaccaacaa tttaaaaagc
     1201 tattgggctt ccacttaggg cggaaataaa tgcccaccta gcaggtctta gctcaaaaaa
     1261 aaacaaaaaa cccaaatttc ttaagtagtc ccaagagaaa aataaatttt agttcactct
     1321 aagatgttaa tgtctactac agaacactag tccactaggg tttttttcag tatcatttct
     1381 caggcacatc ttagttttct ttagaggacc cagagccgga agtgcttccc ttttctctgc
     1441 taggacctaa gactccgtcc catcatacct tgcgcgccgc cgccgcctct tccttcttcc
     1501 tcggcgctgc ctacgaggtg gctgccatct gttttacggt gagtctgtat cggcggccat
     1561 ccgccgcccg cggcttcgac gggaccccgt ctccgggtgg ggaggcctcg gcggcggcct
     1621 ccgcaccagc gtggtagtgg cgagcgcgcg cgggcctgcc actggcgctg ctccggagtc
     1681 tgcccgggtc ggcatcggcc agggcgggct tgcgccatgc ggcccgagag tttcggacgg
     1741 cacccgccgc tttctgctta gcttgttagc agcctttttc tgcggcgaat ccgaaatctt
     1801 aagtcaggga gctgccgccg ctcgccagtg agcagaaatg ctttccgaag cgcaagcccg
     1861 gcggagcagg ttgtctgcgc ggggagcaga gaaggcaccc tttggttggt gctctgagac
     1921 gaccgggagg gacagacgga ctggtggcgt gttaggagtg catggtggct ttgcgtattt
     1981 aagggcgcaa cacttcaaac ataacgaagc ttagtggagc gggatttgcc gccctcgcca
     2041 ccagagagac ctggctgggg ttctgtaaga tcggttgcgg ttctttgagg ttcggagtgg
     2101 gtgagatgaa ccactgacct cttgggatcg cgtctggaga gtagctagta ttcttcgagc
     2161 ttcggaagca agagggagag caagcctagc agaggtaccc atttcatttc cagtttgctc
     2221 ggtagctggt gattggaaga cactctgcaa cattactcac tgaaggcctg agacgcagcc
     2281 ccacctccca gtttctttac taacttgggt ctgacttttg tcaaaaggca tcatggctgc
     2341 cctccggcct ctggtgaagc ccaagatcgt caaaaagagg accaagaagt tcatcaggca
     2401 ccagtcagac cgatatgtga aaattaaggt atgtggttct ggggtggggg ttaagacctg
     2461 agcacacatt tctcgttatc gcttgtctct gcattttaaa aagttcgttt taaagatgac
     2521 actttgtttt atatttatgc gttcgaaggg cagggtgaat cactttaaag gagtggattc
     2581 tctccttcct gtcttgtggt gtccaggtca gatttagttt gtctgacagc aggcactttg
     2641 aagtcatctc tgcaccctga ccctgtattt gaaagatctt tggggttttg tctgcacacg
     2701 caggcacatg catgcatgga attgaacctc tggaagagca gccagtgctc ttaactgcta
     2761 agacctctct ctgggcctgc agatcacctg ttggctggat gggcatgcac acccagcagt
     2821 tcagcagtta actgatccct cctccctact taattgcaat ggaagatgat gttgaaattc
     2881 cagtgcaagt gctagggatg gagcccaggg ccctgtgcat gccaggcaga cactgctaac
     2941 tgagctacac ctcggccttt ggtgtgtgtt ttatgacatg acccccttga attctgtccc
     3001 cttcctgcac acacatacaa gagctgccag tttgcacaga gactaacttg cctgtgtgtc
     3061 ttcagcgaaa ctggcggaaa cccagaggca ttgacaacag ggtgcggaga aggttcaagg
     3121 gccagatcct gatgcccaac atcggttatg ggagcaacaa gaaaaccaag cacatgctgc
     3181 ccagcggctt ccgcaagttc ctggtccaca atgtcaagga gctggaggtg ctgctgatgt
     3241 gcaacaagtg agttgggccc ctggctggga gtgtgcctgc ctcctgtgag actgaggcca
     3301 ctgcagtggg catgcttatg aggaaagagg acgtgttccc ttggttgaag cctgagactg
     3361 gagaagaagg tctttttaca gtgggtgttc agtgtccttt atggtcctgt gtggaacact
     3421 gctatagtgt cacgagactg caggggcctg tgtctaacct aggacctaag ctgtgcttgg
     3481 ctctgctctg aacgggtgga tttgcagagc agctcaggtc ttttggggct gaggtagaga
     3541 aaaagtctcc acgcacagct gtagagagaa ggttgtgggg tattctgggt actttgagca
     3601 agttacaacc cttggtgacc tctgagatgc aaaaccaagg cctctcagga aatactcttg
     3661 acattggata tgagggtgtg atggaaatag tttatttttc ccaagtaaac taggactgca
     3721 gagagtgtat gtgttcgggg ttataaggat ttgttttcta aggtggttgt catgcagatg
     3781 gagaggagtc ctatggggag attatgttgt gcatttcaca aggccttgaa gtgctacaag
     3841 ttccctttgg aaggatggat gcaggagaga gaatgtgtgt tgtacagcag cagtccatga
     3901 ggcagtgttg atggtgcagt gttatgggca ctgcatttct aatgggtaca tctgccctag
     3961 gaatagctct tggccttgtc agaccctgca ggtgcaccgc tcactgagtt ctgtctctgg
     4021 gcattttcct ctgtatcagc taccgagcag ggcaagttgc agggtagtta cagaaaaacc
     4081 attggttcct gagtggtaaa gagtcctggg gtgggatatg gcagcatgtg acttgtgaat
     4141 ggtgagtgtg ctagacagct ataaatgcat gtggccccgg gatgggtgct tccggttaga
     4201 acgtgatgca ctctgtcccc aaatctgtca ggtggtgtct gtctgtatta ggtatttgcc
     4261 atttgtatgc tttccatagc tctgccatcc tgcttctcta ggggtttttc ctcagctccc
     4321 ggtctatggc ttcacggtca tctccatgct gggaatgtgt tcccttagga cagattgtca
     4381 acactttgag ggaacacggg cagtgtttgt tgttcacgct gcttgaactg tgtaccttac
     4441 agtggttgca ctggaagcct ttggctccag ttggaagaat tctgggttac tcagtgcgac
     4501 gacgaattaa gcgatttccc tggtgacctt tctggtttgc ttctctttca gatcttactg
     4561 tgctgagatt gctcacaatg tgtcctctaa gaaccgaaaa gccattgtag aaagagcagc
     4621 acagctggcc atcagagtca ccaatcccaa cgccaggcta cgcagcgaag aaaatgagta
     4681 gatggcttgt gtgcatgttt tatgtttaaa taaaatcaca aaacctgccg tcgtattttc
     4741 tatgtttttg gtaactggag attgttcttt gctgcttgtt ctgggggaga cagcagggtt
     4801 cagaatccca tgctagtctt ggttggctgc aggtggaaga ggggaaaggc cttgaccctc
     4861 tacagtgtag gtacacttag agtctgggga ccagacaaac ctctgctctc aaccctgtcc
     4921 caactctagc atgggctcta gcttaaggca cttttttttt tttttttttt tttttgtttt
     4981 tcgagacagg gtttctctgt atagtcttgc ctgtcctgga actcactttg tagaccaggc
     5041 tggcctcgaa ctcagaaatc tgcctgtctc tgcctcccaa gtgttgggat taaaggcgtg
     5101 cgccaccact gcccggctta aggcactttt ttgatgaagc ctttagtttc atggaactgg
     5161 cctgcttggc ctcaatcagt caggattgac aatcctgtct ttggaggata ttagaacttc
     5221 atgagactga taaatacttc tgccacgtgg gacactacaa gggcagtgcc atctgtatct
     5281 aggccaatct ctggctcgct gttctttgga aacagtaatg gagttcggaa gggttgactg
     5341 tcacgggtga cacttaaatg actggagaga aagacatccc 
//
K02061. Mus musculus L32-...[gi:3228368]
GenBank Entry
LOCUS       MUSRPL4A                1516 bp    DNA     linear   ROD 16-JUN-1998
DEFINITION  Mus musculus L32-4A pseudogene, complete sequence.
ACCESSION   K02061
VERSION     K02061.1  GI:3228368
KEYWORDS    .
SOURCE      house mouse.
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Rodentia; Sciurognathi; Muridae; Murinae; Mus.
REFERENCE   1  (bases 1 to 1516)
  AUTHORS   Dudov,K.P. and Perry,R.P.
  TITLE     The gene family encoding the mouse ribosomal protein L32 contains a
            uniquely expressed intron-containing gene and an unmutated
            processed gene
  JOURNAL   Cell 37, 457-468 (1984)
  MEDLINE   84205680
REFERENCE   2  (bases 528 to 1234)
  AUTHORS   Antonov,L.S.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-1993) Molecular Genetics, Institute of Genetic
            Engineering, Kostinbrod-2, Bulgaria
REFERENCE   3  (bases 1 to 1516)
  AUTHORS   Antonov,L.S.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JUN-1998) Molecular Genetics, Institute of Genetic
            Engineering, Kostinbrod-2, USA
  REMARK    Nucleotide sequence updated by submitter
COMMENT     On Jun 16, 1998 this sequence version replaced gi:200782.
            The processed gene rpL32-4A seems to be highly conserved. It has
            been preserved in the C3H strain and has evolved to its present
            form in BALB/c mice by a single 'a' to 'g' substitution.
FEATURES             Location/Qualifiers
     source          1..1516
                     /organism="Mus musculus"
                     /strain="BALB/c"
                     /db_xref="taxon:10090"
                     /clone="rpL32-4A"
                     /cell_type="sperm"
     repeat_region   317..323
                     /rpt_family="B2"
                     /rpt_type=terminal
     repeat_region   328..548
                     /rpt_family="B2"
                     /rpt_type=dispersed
     repeat_region   574..587
                     /rpt_family="4A"
                     /rpt_type=terminal
     mRNA            589..1094
                     /product="L32-4A"
     CDS             640..1047
                     /note="L32-4A"
                     /codon_start=1
                     /pseudo
     repeat_region   824..967
                     /rpt_family="B2"
                     /rpt_type=terminal
     repeat_region   1121..1134
                     /rpt_family="4A"
                     /rpt_type=terminal
     repeat_region   1207..1280
                     /rpt_family="B1"
                     /rpt_type=dispersed
BASE COUNT      438 a    355 c    342 g    381 t
ORIGIN      
        1 aggaaccaag actcaggttt gtcacacaca acactattct cctatggcac aggaaaataa
       61 cactaacaca ttcttggatt tggaccctac tttttcaaga ttccttgcaa cctctccttt
      121 tgttatgaca acacagcact tacttcatcc tcattacagc tgaactgaga tagctctccc
      181 tgcccccagt gaattgctga atcccactgc agttgtttgt acttcaggac tggcctggcc
      241 gagctcaggc agatcctgtt aggactgtgc tgagaggata tgcagtctca tctcagaact
      301 cgcagggaaa tgctcgagga acactacttt tttaaagatg tagtcatttt ttattttatg
      361 tgtttggctg tttcgcctgc atgtatgtat gtgtgctatg tgtgtctggt gctcgtggaa
      421 acgagaagaa ggtgtcacac cctctggaac tacagttaca gagagttgtg aggcaccgtg
      481 tgggtgcttc ggaactctgt aggagcagtg gagtgctctt caccactgaa tcatccctcc
      541 agtatcccaa gtaacatcat tataatacac attacaaatt agctgctgct tcttcctcgg
      601 cgctgcctac gaggtggctg ccatctgttt tacggcatca tggctgccct ccggcctctg
      661 gtgaagccca agatcgtcaa aaagaggacc aagaagttca tcaggcacca gtcagaccga
      721 tatgtgaaaa ttaagcgaaa ctggcggaaa cccagaggca ttgacaacag ggtgcggaga
      781 aggttcaagg gccagatcct gatgcccaac atcggttatg ggagcaacaa gaaaaccaag
      841 cacatgctgc ccagcggctt ccgcaagttc ctggtccaca atgtcaagga gctggaggtg
      901 ctgctgatgt gcaacaaatc ttactgtgct gagattgctc acaatgtgtc ctctaagaac
      961 cgaaaagcca ttgtagaaag agcagcacag ctggccatca gagtcaccaa tcccaacgcc
     1021 aggctacgca gcgaagaaaa tgaatagatg gcttgtgtgc atgttttatg tttaaataaa
     1081 atcacaaaac ctgcaaaaaa aaaaaaaaaa aaaaaaaaaa acaaattagc tgcttttttt
     1141 tcataatccc cattgcctct tgattatttt ccttttgagg tttgagacaa gctctcactg
     1201 tacatgtcaa actcataaag atgccgttga ctctgcctcc cgagtgccgt gattaaaggc
     1261 atgtgccacc accgcctatc aagggtcatt ttcaaaagct ccacttggaa aatctatgtc
     1321 atacgtaagt gataaggcaa gtgcaaggtc acagacgttt ccacctcaag cttcacaaga
     1381 gaatcctgag agaccaagtg atgcaaagca agatcagaat ccctagggcc atttttgaga
     1441 gagcagtgtt tgcagctatg ggtgaaactg aagatgaagt agcgcctgcg aagacattgg
     1501 aagatgccag gaattc
//